Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications

Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications

We describe an optical system which reduces the cost and complexity of fluorescence correlation spectroscopy (FCS), intended to increase the suitability of the technique for clinical use. Integration of the focusing optics and sample chamber into a plastic component produces a design which is simple to align and operate. We validate the system by measurements on fluorescent dye, and compare the...

Intracellular applications of fluorescence correlation spectroscopy: prospects for neuroscience.

Based on time-averaging fluctuation analysis of small fluorescent molecular ensembles in equilibrium, fluorescence correlation spectroscopy has recently been applied to investigate processes in the intracellular milieu. The exquisite sensitivity of fluorescence correlation spectroscopy provides access to a multitude of measurement parameters (rates of diffusion, local concentration, states of a...

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy.

This article focuses on methods based on fluctuation correlation spectroscopy to determine the formation of protein complexes in living cells. We present the principles of the fluctuation method applied to cells. We discuss the novelty and the promises of this approach. The emphasis is in the discussion of the underlying statistical assumptions of the image correlation spectroscopy analysis rat...

Biological and chemical applications of fluorescence correlation spectroscopy: a review.

The mathematical concept of fluorescence correlation spectroscopy (FCS)1 (1) emerged from quasi-elastic light scattering (QELS) spectroscopy (2) in the early 1970s. Compared to light scattering, the enhanced sensitivity of fluorescence to changes in molecular structure, chemistry, and local environment makes FCS a superior analytical tool for chemical kinetics studies (1, 3-5). The primary moti...

Fluorescence correlation spectroscopy.